Fourier transform infrared spectrscopy and the amide region

 Are there organics in microbial biofilm that could interfere with the amide region of FTIR spectrum?

Infrared spectroscopy is one of the oldest and well established experimental techniques for the analysis of secondary structure of polypeptides and proteins.

Typical Amide I and Amide II peaks in FTIR spectrum (Fig. 1 from Kong and Yu, 2007).

 

Table 4 from Kong and Yu, 2007

Kong and Yu, 2007, Fourier Transform Infrared Spectroscopic Analysis of Protein Secondary Structures, Acta Biochimica et Biophysica Sinica, 39(8): 549–559